Chapter 7 The Microscope 1 Physical properties of

Chapter 7 The Microscope 1 Physical properties of

Chapter 7 The Microscope 1 Physical properties of light The Compound

Microscope Schematic diagram of stereoscopic microscope Two separate monocular

microscopes Each has own set of lenses except for the lowest Objective lens common to both microscopes

3 The Compound Microscope Condenser: lens system under the microscope stage that focuses light onto the specimen

4 The Comparison Microscope Two independent objective lenses joined together by an optical bridge to a common eyepiece lens.

The objects under investigation are observed side-by-side in a circular field that is equally divided into two parts Modern firearms examination Began with the introduction of the comparison microscope Ability to give the firearms examiner a

side-by-side magnified view of bullets 5 Comparison Microscopes Are used to compare two specimens Consist of two compound microscopes connected by an optical bridge

Provide a single eyepiece through which the examiner sees both images side by side Can be lighted from below the stage or via a vertical or reflected illumination system The Comparison Microscope

7 The Stereoscopic Microscope Two monocular compound microscopes

Three-dimensional image of a specimen Use both eyepiece lenses Evidence not requiring very high magnification (10x125x) Large working distance Microscopic examination of big,

bulky items 8 The Stereoscopic Microscope 9

Double-refraction in Calcite: -the secret key to PLM Polarizing microscopy Birefringence Calcite produces two images when it is placed over the blue pencil. One of the images appears normally as when viewing an object

through clear glass. The other pencil image appears displaced, due to the nature of doubly-refracted light. When anisotropic crystals refract light, the resulting rays are polarized and travel at different velocities. One of the rays travels with the same velocity in every direction through the crystal and is termed the ordinary ray. The f = V/w f-frequency

V-velocity of light w-wavelength Polarizing microscopy Polarized light Polarizing Microscopy

Plane-polarized Light that is confined to a single plane of vibration Polarizing microscope Examination of the interaction of plane-polarized light with matter

14 Polarizing Microscopy Wide applications for the study of birefringent materials Materials that split a beam of light in two

Each with its own refractive index value Determination of these refractive index data Provides information that helps to identify minerals present in a soil

sample or the identity of a man-made fiber. 15 Polarizing Microscopes Polarizing microscopes Include two polarizing filters, a polarizer lens

(fixed below the specimen), and an analyzer lens (fixed above the specimen) The stage with the sample is rotated to determine how the polarized light interacts with the sample Polarizing microscope Polarizer Analyzer

Polarizing Microscopes This can provide information about the shape, color, and size of minerals and it is used to identify hair, human-made fibers and paint. Hair Sample: Natural Light

Hair Sample: Polarized Light Michel-Levy Chart of Birefringence f = V/w f-frequency V-velocity of light

w-wavelength f is nearly always constant, therefore is V changes, w must change as well. n (Refractive Index)

= c/v c-speed of light in vacuum When light crosses boundary of 2 materials with different refractive indices [n], it is bent Polarizing microscopy

Analyzer out of path = plane polarized light; gives specimens true color Analyzer in path = crossed polars; gives specimens interference Polyester,

color Polyester, plane-polarized light under crossed polars Polarizing microscopy Improves specimen contrast

Stained plant fibers, left stained; right polarized light Polarizing microscopy Many crystalline substances are birefringent Forensic analysis of

soil components Many synthetic fibers are birefringent Refractive Index by the Becke Line

Method Note: when refractive index of mineral & liquid are close, relief if low. Plagioclase Feldspar showing albite twinning -used to determine Plagioclase composition

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