Corn genetics Lab - Gulf Coast State College

Corn genetics Lab - Gulf Coast State College

Corn Genetics & Chi- Square Goodness of Fit Test Pedigrees and probability analysis to follow.

Outline Highlight some key points in syllabus Corn color and collection of data (phenotypes)

Chi-Square Analysis Corn genetics Lab Application of Chi-Square Analysis

The color pattern in corn is due to multiple genes Are variations in number of observed phenotypes due to chance alone? Objective

Complete corn worksheet Set up Punnett Squares using the genotypes given Compare expected data to observed data Perform Chi Square Analysis

Variation of colorsWhy? Corn Kernel Color Color of aleurone masks endosperm color Only with colorless aleurone can endosperm color be determined

Determined by several genes Refer to genotypes in lab manual! Gene interaction can occur: Epistasis is a form of gene interaction in which one gene masks the

phenotypic expression of another. Today there are: 3 crosses of corn F1 X F1 Multiple alleles are tested for expression in F2 generation

Note color differences Cross C Cross D (darker color is purple) Cross F (call purple, may look red!)

Cross C F1 phenotype F1 X F1 genotypes Yellow

YYCRr X YYCRr Cross D F1 phenotype F1 X F1 genotypes

Purple yyPpRr X yyPpRr Cross F F1 phenotype F1 X F1 genotypes

Purple yyCcRr X yyCcRr Chi Square Statistical Goodness of Fit Test

Why do we use it? To find out determine . if probability are due to chance alone.

An example of how to calculate a Chi Square Application of Chi Square Genetic information regarding the corn color patterns are provided in your lab manual.

CORN WORKSHEET IS DUE 3/5

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