# Class details - University of California, Irvine

Last class Gel filtration PAGE SDS vs Native The MM equation

Assumptions: v0 is _________ reaction rate _____________ velocity Measured at ____________: Formation of ES = Breakdown ES [S] >>>>of[E]

Measured at given ________ What do the terms mean? v0 v0 is _________ reaction rate Measured at given [S] and given [E]

What do the terms mean? Vmax Vmax is _____________________ reaction rate Reaction rate when [ES] = _________

Vmax = k2 * __________ Can it ever be practically achieved? What do the terms mean? Km [S] V 0 = V Max

[ S ] + Km [S ] [ S ] + Km = VMax V0 [S ] [ S ] + Km = VMax

0.5 V Max [ S ] + Km = 2[ S] Km = What do the terms mean? Km

Km is _____________ of enzyme for substrate It is not 2 * Vmax! The plot thickens Practical considerations

How to find Km and Vmax for an enzyme? Practical considerations Asymptote Reproducible? Solution?

Better method: LWB plot Asymptote Reproducible? Solution? Better method: LWB plot Lab

Fill out tables correctly! Change [E] Effect on rate of reaction? Calculate K for different m substrates

- Get rate over time using different [S]each reaction rate - Get v for 0 (how?)

- MM and LWB plots - Look over data! Studying proteins What are things that you might want to know about a protein?

Studying proteins How would you study a protein? Studying proteins How to tell what a protein is need for?

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